Se diseñaron los biorreactores, utilizando principios de similitud; para la producción del alcohol etílico, se diseñó un biorreactor del tipo Lecho Empacado . Inmovilización de levaduras en residuos lignocelulósicos para la producción de etanol en biorreactor de lecho empacado. En las fermentaciones realizadas en los biorreactores de lecho empacado con el biocatalizador (soporte + levaduras), se logró obtener un aumento en la.

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Biology of Microbial Communities – Interview.

Yeast immobilization in lignocellulosic wastes for ethanol production in packed bed bioreactor

It is observed that the cane bagasse was the material in which the highest amount of cells was immobilized, 0. Enzyme Microbiology and Technology. In the last two decades there has been an increase of research focused on developing new technologies for ethanol production from renewable sources, and the efforts have focused towards the development of much more efficient production processes [1- 3]. Preparar 5 L de influente en total para poner en marcha el reactor. There are also demanding requirements for materials used as carriers, since chemical, physical and biological stability has to be ensured, and the carriers and the technique must be low cost and easy to implement on an industrial scale.

Ethanol production began in the early hours of fermentation, but only provided an exponential accumulation by 6 hours after the process started. The ethanol concentration was determined by HPLC, by analyzing samples of the effluent of the reactors.


Revista Facultad de Ingeniería

The main objective of the present research seeks to design two types of bioreactors for producing metabolites of ethylic alcohol and citric acid at the laboratory. The fermentations with glucose as the carbon source were performed using cane bagasse as the carrier.

However, these values are higher than the ones obtained in biorreacotr fermentation with glucose. To establish immobilization capacity on each of the materials and determine the effect of size and flow rate in the process, we standardized the biomass quantification methodology on the carriers.

Un biorreactor Novela de alta densidad de cultivo de diversas comunidades microbianas

We can see that the amount of immobilized cells varies depending on the treatment and the material. Critical Reviews in Biotechnology. The main mechanisms that occur during immobilization by adsorption are: We obtained a consumption of An important result obtained from the experimental design, is about selection of the lechl material for cell immobilization.

We obtained pieces of material of sizes between 3.

Modelado de reactores de lecho empacado en COMSOL®

We took the sample at the end of the fermentation. We made continuous fermentation using the biocatalysts obtained with the four materials.

After drying, they were weighed again. This Acid was analyzed and identified by Spectroscope method called Ultra Violet Visible corroborating its existence. Preparar el inoculante algas. Empacaeo shown in the results of table 3, it is the cane bagasse the material on which the biggest quantity of biomass was immobilized. Some of the disadvantages of these processes are: The same process was implemented with cell-free material to be used as blank. Among the most widely used immobilization matrices are hydrophilic polymer gels such as biorreator, carrageenate, agarose, etc.


Instale los cabezales de bomba en las unidades de bombeo con la provi tornillosded con las unidades de bombeo.

You must be signed in to post a comment. Continuous processes with immobilized cells are a good alternative for improving the efficiency and the performance of borreactor fermentations. Used as mixing vessel MV.

Analytical methods Microbial biomass was determined by the dry weight modified protocol developed in this research. We have also established that the glucose syrup obtained from the enzymatic hydrolysis of cassava flour can be used empacxdo an alternative substrate in alcoholic fermentations.

Biotechnology and Bioengineering 64, pp. The filter attached to the reactor output was used to retain the cells that were not em;acado within the process. These results are similar to the values obtained with the sucrose.

De-inmobilization was performed by mechanical agitation at rpm for 24 hours at room temperature.